IKK alpha antibody (pThr23)

Catalog No. ABIN1531873

This Rabbit Polyclonal antibody specifically detects IKK alpha in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IKK alpha antibody (pThr23) (ABIN1531873)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IKK alpha Antibody

Binding Specificity: AA 15-64, pThr23

Specificity:

IKK-alpha (Phospho-Thr23) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at Thr23.

PhosphorylationH:T23 M:T23 R:T23

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.198998 (NCBI Gene Symbol: CHUK)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK-alpha

Background:

Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkapp

NCBI Gene Symbol: CHUK

Molecular Weight: 84 kDa

Gene ID: 1147

OMIM: 600664

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins