Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.
Cross-Reactivity (Details)
Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody reacts with mouse V-ATPase subunit A. Sequence homology predicts that it will also react with human, bovine, orangutan, tobacco hornworm, chicken, fruit fly, and zebrafish V-ATPase subunit A.
ATP6V1A
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/mL Western blot: 1.0 µg/mL Other applications: user-optimized
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freezing and thawing cycles.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.
Gokey, Dasgupta, Amack: "The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish." in: Developmental biology, Vol. 407, Issue 1, pp. 115-30, (2016) (PubMed).
Grubman, Lidgerwood, Duncan, Bica, Tan, Parker, Caragounis, Meyerowitz, Volitakis, Moujalled, Liddell, Hickey, Horne, Longmuir, Koistinaho, Donnelly, Crouch, Tammen, White, Kanninen: "Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder." in: Acta neuropathologica communications, Vol. 2, pp. 25, (2014) (PubMed).
Monteiro, Aires, Becker, Jacinto, Certal, Rodríguez-León: "V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration." in: PLoS ONE, Vol. 9, Issue 3, pp. e92594, (2014) (PubMed).
Li, Chen, Klemmer, Koopmans, Karupothula, Smit: "Identifying true protein complex constituents in interaction proteomics: the example of the DMXL2 protein complex." in: Proteomics, Vol. 12, Issue 15-16, pp. 2428-32, (2012) (PubMed).
Nylandsted, Becker, Bunkenborg, Andersen, Dengjel, Jäättelä: "ErbB2-associated changes in the lysosomal proteome." in: Proteomics, Vol. 11, Issue 14, pp. 2830-8, (2011) (PubMed).
Target
ATP6V1A
(ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))
The V-ATPase subunit A is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.Rabbit Anti-V-ATPase Subunit A Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide, purified from rabbit antiserum by immunoaffinity chromatography, and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.