Huntingtin antibody (C-Term)

Catalog No. ABIN1607891

Product Details anti-Huntingtin Antibody

Target: Huntingtin (HTT)

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with HTT from other sources has not been determined.

Purity: Anti-Huntington was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Huntington.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-Huntington antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 0.1 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: Huntington (HTT Products)

Synonyms: HTT antibody, hd antibody, ZHD antibody, CG9995 antibody, Dmel\\CG9995 antibody, HD antibody, Hsap\\HD antibody, Htt antibody, dHtt antibody, dhtt antibody, SLC6A4 antibody, huntington antibody, it15 antibody, htt antibody, IT15 antibody, AI256365 antibody, C430023I11Rik antibody, Hd antibody, Hdh antibody, huntingtin antibody, HTT antibody, htt antibody, LOC373520 antibody, Htt antibody

Background: Huntington's disease (HD) is an autosomal dominant neurological disorder caused by a polyglutamine (polyQ) repeat expansion in the huntingtin (Htt) protein. The disease is characterized by neurodegeneration and formation of neuronal intracellular inclusions primarily in the striatum and cortex, leading to personality changes, motor impairment, and dementia. The Huntington’s disease protein is ~350kD in size and is localized in the brain. Anti-Huntingtin antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research. Synonyms: HD protein, HTT, huntingtin, huntingtin (Huntington disease), IT15HD, Huntington disease protein

Gene ID: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport