FZR1 antibody (AA 141-240) (HRP)

Catalog No. ABIN1711687

Product Details anti-FZR1 Antibody (HRP)

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Binding Specificity: AA 141-240

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FZR1/CDC20C

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: FZR1/CDC20C (FZR1 Products)

Synonyms: CDH1-C antibody, FYR antibody, FZR antibody, CDH1 antibody, fzr1 antibody, CDC20C antibody, FZR2 antibody, HCDH antibody, HCDH1 antibody, AW108046 antibody, Cdh1 antibody, Fyr antibody, zgc:55450 antibody, fizzy and cell division cycle 20 related 1 antibody, fizzy/cell division cycle 20 related 1 antibody, fizzy-related protein homolog antibody, fizzy/cell division cycle 20 related 1 (Drosophila) antibody, fizzy/cell division cycle 20 related 1a antibody, Fzr1 antibody, CDH1-C antibody, FZR1 antibody, LOC100027495 antibody, LOC100179951 antibody, fzr1a antibody

Background:

Synonyms: FZR, FZR2, CDC20C, Fizzy-related protein homolog, CDC20-like protein 1, Cdh1/Hct1 homolog, FZR1, FYR, KIAA1242

Background: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Gene ID: 51343

UniProt: Q9UM11

Pathways: DNA Replication, Synthesis of DNA