EPOR antibody (pTyr485)

Catalog No. ABIN1713625

Product Details anti-EPOR Antibody

Target: EPOR (Erythropoietin Receptor (EPOR))

Binding Specificity: pTyr485

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human EPO Receptor around the phosphorylation site of Tyr485

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EPOR

Target: EPOR (Erythropoietin Receptor (EPOR))

Alternative Name: EPO Receptor (EPOR Products)

Synonyms: EPOR antibody, epor antibody, EPO-R antibody, erythropoietin receptor antibody, erythropoietin receptor L homeolog antibody, EPOR antibody, epor antibody, Epor antibody, epor.L antibody

Background:

Synonyms: EPO Receptor phospho Y485, p-EPO Receptor phospho Y485, EPOR phospho Y485, p-EPOR phospho Y485, erythropoietin receptor, EPO R, EPO Receptor, Erythropoietin receptor precursor.

Background: The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

Gene ID: 2057

Pathways: JAK-STAT Signaling