GFP antibody
(1 reference)-
- Target See all GFP Antibodies
- GFP (Green Fluorescent Protein (GFP))
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Reactivity
- Aequorea victoria
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GFP antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC)
- Cross-Reactivity (Details)
- Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Strep-Tag® fusion protein, full-length GFP.
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea aequorea WB, IF, IHC (fro), IHC (p) Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP WB, ELISA, IF, IP, FACS Host: Mouse Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP Reactivity: Aequorea victoria WB, ELISA Host: Mouse Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC
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- Application Notes
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WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet - Comment
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ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
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: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, (2004) (PubMed).
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- Target
- GFP (Green Fluorescent Protein (GFP))
- Alternative Name
- GFP (GFP Products)
- Synonyms
- green fluorescent protein antibody, gfp antibody
- Target Type
- Viral Protein
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