GAD65 antibody (AA 3-96)

Catalog No. ABIN1742419

Product Details anti-GAD65 Antibody

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Binding Specificity: AA 3-96

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Specific for GAD 2 / GAD 65.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant mouse GAD 2 (aa 3-96).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 2000
IHC: 1 : 1000

Comment:

ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-GAD65 antibody (ABIN1742419)

Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: "Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).

Saliba, Gu, Yan, Moss: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, (2009) (PubMed).

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD 2 (GAD2 Products)

Synonyms: GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody

Background: Synonyms: GAD 65