MUC1 antibody

Catalog No. ABIN1804118

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Binds with high efficiency to an epitope within the VNTR recombinant repeat peptide region of MUC1 Molecule. Reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (but not with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1, but not with completely glycosylated MUC1. Belongs to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non cross-reactive with normal serum proteins and human tissues of nonepithelial origin.

Purification: Protein A purified

Immunogen: High molecular weight (>300kD) glycoprotein from human milk-fat globule molecule.

Clone: M4H2

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (10 μg/mL)

Usage: Immunohistochemical and immunofluorescent detection of neoplastic breast epithelium. Works on frozen tissue sections. Construction of immunometric assay for MUC determination. ELISA tests for measurement of unglycosylated and underglycosylated tumor-associated MUC1mucins in human serum. Detection of unglycosylated MUC1. Can be used for immunohistochemical staining of paraffin and frozen sections of breast, lung and ovarian tumor tissues. Staining of paraffin-embedded tissue sections fixed in neutral-buffered formalin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: EMA / MUC1 (MUC1 Products)

Synonyms: CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody

Background: Name/Gene ID: MUC1
Family: Mucin

Synonyms: MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane

Gene ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling