ELISA. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Western Blot: Under non-reducing conditions a PSA triplet is observed at approx 30 kDa. Under reducing conditions a doublet can be observed. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.2, 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
PSA is a serine protease produced by prostatic epithelial cells, which is also known as kallikrein 3. Serum PSA levels have been correlated with the stage of prostatic cancer, and have been used to monitor residual tumour levels and disease progression.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase