IFNB1 antibody (Biotin)

Catalog No. ABIN181768

This Rabbit Polyclonal antibody specifically detects IFNB1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for IFNB1 antibody (Biotin) (ABIN181768)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IFNB1 Antibody (Biotin)

Specificity: This antibody recognizes IFNB / Interferon beta.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human IFN beta

Application Details

Application Notes: Direct ELISA: To detect Human IFN beta by Direct ELISA (using 100 μL/well antibodysolution) a concentration of at least 1.0 μg/mL of this antibody is required. ThisBiotinylated antibody allows the detection of 0.2-0.4 ng/well of recombinant Human IFNbeta. Sandwich ELISA: To detect Human IFN beta by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody in conjunction with Anti Human IFN beta antibody (PP1230P1 or

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Alternative Name: IFNB / Interferon beta

Background: The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50 % amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1

Gene ID: 15977

NCBI Accession: NP_034640

UniProt: P01575

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome