MTOR antibody (Ser2448)
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- Target See all MTOR (mTOR) Antibodies
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Binding Specificity
- Ser2448
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This MTOR antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- Specificity
- Endogenous levels of human mTOR only when phosphorylated at Ser2448. Species Crossreactivity: mouse, rat.
- Purification
- Purified
- Immunogen
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Synthetic peptide corresponding to residues surrounding Ser2448 of human mTOR.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product mTOR Primary Antibody
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- Application Notes
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Approved: IF (1:50), IP (1:50), WB
Usage: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4 °C with gentle shaking, overnight. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, less than 0.02 % sodium azide, 50 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
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- Target
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- Alternative Name
- MTOR (mTOR Products)
- Synonyms
- FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody
- Background
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Name/Gene ID: MTOR
Subfamily: PI3/PI4
Family: Protein Kinase
Synonyms: MTOR, FRAP1, FRAP2, FRAP, Mammalian target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, RAPT1, Rapamycin target protein 1 - Gene ID
- 2475
- UniProt
- P42345
- Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
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