PRKAA1/PRKAA2 antibody (pThr172, pThr174)

Catalog No. ABIN1842476

Product Details anti-PRKAA1/PRKAA2 Antibody

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Binding Specificity: pThr172, pThr174

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: AMPKalpha,1/AMPKalpha,2 Antibody detects endogenous levels of total AMPKalpha,1/AMPKalpha,2 protein.

Characteristics: Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa

Purification: Rabbit Anti-AMPKalpha,1/AMPKalpha,2 Antibody (Ab-174/172) Polyclonal Antibody was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from human AMPKalpha,1/AMPKalpha,2 around pThr174,pThr172 (L-R-TP-S-C).

Isotype: IgG

Application Details

Application Notes: IHC: 1:50-1:100,
WB: 1:500-1:1,000,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

References for anti-PRKAA1/PRKAA2 antibody (ABIN1842476)

Arduini, Serviddio, Escobar, Tormos, Bellanti, Viña, Monsalve, Sastre: "Mitochondrial biogenesis fails in secondary biliary cirrhosis in rats leading to mitochondrial DNA depletion and deletions." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 301, Issue 1, pp. G119-27, (2011) (PubMed).

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Abstract: PRKAA1/PRKAA2 Products

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody

Background: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.