PRKAA1/PRKAA2 antibody (Thr172, Thr183)

Catalog No. ABIN6280795

Product Details anti-PRKAA1/PRKAA2 Antibody

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Binding Specificity: AA 110-190, Thr172, Thr183

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: AMPKα1/2 is a protein encoded by the PRKAA1 gene which is approximately 64 kDa. AMPKα1/2 is localised to the cytoplasm and nucleus. It is involved in RET signalling, mTOR signalling, AMP-activated protein kinase signalling, the regulation of TP53 activity and longevity regulating pathway. It is a catalytic subunit of AMP-activated protein kinase, an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, it activates energy-producing pathways and inhibits energy-consuming processes such as protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPKα1/2 is expressed in nervous system, liver, blood, intestine and kidney. Mutations in the PRKAA1 gene may result in obesity. STJ91581 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of AMPKα1/2 protein.

Specificity: AMPKα1/2 Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.

Characteristics: Rabbit polyclonal to AMPKα1/2.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AMPKalpha1/2 around the non-phosphorylation site of T183/172.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Alternative Name: AMPKalpha1/2 (PRKAA1/PRKAA2 Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, Ampk antibody, Ampka2 antibody, 2310008I11Rik antibody, A830082D05 antibody, AMPKalpha2 antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody, PRKAA2 antibody, Prkaa2 antibody

Molecular Weight: 63 kDa

Gene ID: 5562

UniProt: Q13131