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GFP antibody

GFP Reactivity: General WB, ELISA, IHC, FACS Host: Mouse Monoclonal unconjugated
Catalog No. ABIN1843725
  • Target See all GFP Antibodies
    GFP (Green Fluorescent Protein (GFP))
    Reactivity
    • 175
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    • 15
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    • 1
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    General
    Host
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    Mouse
    Clonality
    • 121
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    Monoclonal
    Conjugate
    • 103
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    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
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    • 1
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    This GFP antibody is un-conjugated
    Application
    • 172
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purification
    Purified antibody
    Immunogen
    Purified recombinant fragment of GFP expressed in E. coli.
    Isotype
    IgG2a
    Top Product
    Discover our top product GFP Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    PBS containing 0.03 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
  • Target
    GFP (Green Fluorescent Protein (GFP))
    Alternative Name
    GFP (GFP Products)
    Synonyms
    green fluorescent protein antibody, gfp antibody
    Target Type
    Viral Protein
    Background
    GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light.GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors.GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.GFP fluorescence is stable under fixation conditions and suitable for a variety of applications.GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.GFP technnology has considerably contributed to a greater understanding of cellular physiology.
    Molecular Weight
    27 kDa
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