IFNB1 antibody (AA 22-186) (Biotin)
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- Target See all IFNB1 Antibodies
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
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Binding Specificity
- AA 22-186
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IFNB1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -MSYDVLRYQ QRSSNLACQK LLGQLPGTPQ YCLEDRMNFE VPEEIMQPPQ FQKEDAVLII HEMLQQIFGI LRRNFSSTGW NETVIKTILV ELDGQMDDLE TILEEIMEEE NFPRGDMTIL HLKKYYLSIL QYLKSKEYRS CAWTVVQVEI LRNFSFLNRL TDYLRN
- Specificity
- It has been selected for its ability to recognize IFNb in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant IFNb expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IFNb conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product IFNB1 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant IFNb (Met22~Asn186) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))
- Alternative Name
- Interferon Beta (IFNB1 Products)
- Synonyms
- IFB antibody, IFF antibody, IFNB antibody, IFN-beta antibody, Ifb antibody, Ifnb antibody, IFNb antibody, interferon beta 1 antibody, interferon beta 1, fibroblast antibody, interferon, beta 1, fibroblast antibody, IFNB1 antibody, Ifnb1 antibody
- Pathways
- JAK-STAT Signaling, TCR Signaling, TLR Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome
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