SPHK1 antibody (AA 148-398)
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- Target See all SPHK1 Antibodies
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
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Binding Specificity
- AA 148-398
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPHK1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Sphingosine Kinase 1 (SPHK1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against SPHK1. It has been selected for its ability to recognize SPHK1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Pig
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Sphingosine Kinase 1 (SPHK1) corresdonding to Leu148~Leu398 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product SPHK1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- SPHK1 (Sphingosine Kinase 1 (SPHK1))
- Alternative Name
- Sphingosine Kinase 1 (SPHK1 Products)
- Synonyms
- CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody
- Pathways
- VEGF Signaling
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