SIGLEC8 antibody (AA 70-336)

Catalog No. ABIN1865984

Product Details anti-SIGLEC8 Antibody

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Binding Specificity: AA 70-336

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIGLEC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)

Specificity: The antibody is a mouse monoclonal antibody raised against SIGLEC8. It has been selected for its ability to recognize SIGLEC8 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) corresdonding to Gly70~Ser336 with N-terminal His and GST Tag

Clone: C1

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.2-2 μg/mL

1:500-5000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SIGLEC8

Target: SIGLEC8 (Sialic Acid Binding Ig-Like Lectin 8 (SIGLEC8))

Alternative Name: Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8 Products)

Synonyms: SAF2 antibody, SIGLEC-8 antibody, SIGLEC8L antibody, SIGLEC8 antibody, sialic acid binding Ig like lectin 8 antibody, sialic acid-binding Ig-like lectin 8 antibody, SIGLEC8 antibody, LOC506364 antibody

Background: SAF2, SIGLEC-8, SIGLEC8L, Sialoadhesin family member 2