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Histone 3 antibody (H3K27me3)

Name: Histone 3 antibody (H3K27me3)
SKU: ABIN1873002
Price: 479.83 USD
Availability: InStock

H3 Reactivity: Human, Mouse, Rat WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Western Blotting (WB) image for anti-Histone 3 (H3) (H3K27me3) antibody (ABIN1873002)

Immunofluorescence (IF) image for anti-Histone 3 (H3) (H3K27me3) antibody (ABIN1873002)

Chromatin immunoprecipitation analysis of various genes from 293 cell line, using H3K27me3 antibody and rabbit IgG.

ChIP

Immunohistochemistry of paraffin-embedded rat brain tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens).

IHC

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (ABIN1873002) at 1:1000 dilution.

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (ABIN1873002).

Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K27 antibody (ABIN1873002) at dilution of 1:200 (40x lens).

IHC

Immunohistochemistry of paraffin-embedded human thyroid cancer using TriMethyl-Histone H3-K27 antibody (ABIN1873002) at dilution of 1:200 (40x lens).

IHC

Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K27 antibody (ABIN1873002) at dilution of 1:200 (40x lens).

IHC

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 antibody (Red)(ABIN1873002) and BrdU antibody (Green)and rabbit IgG.

11 images

Catalog No. ABIN1873002

Datasheet as PDF

Target See all Histone 3 (H3) Antibodies

Histone 3 (H3)
Binding Specificity
59

47

45

42

41

37

36

35

35

35

34

32

31

29

29

29

26

25

23

23

23

22

20

18

18

17

17

16

16

16

16

16

15

14

14

13

13

12

12

12

12

12

11

11

10

10

10

9

9

8
H3K27me3
Reactivity
1400

941

861

59

42

41

41

40

39

35

35

30

29

22

19

6

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
1218

243

8

4
Rabbit
Compatible Secondaries
Clonality
1121

351

1
Polyclonal
Conjugate
822

81

63

63

63

62

62

62

37

33

23

17

17

17

17

17

14

2

1
This Histone 3 antibody is un-conjugated
Application
1059

411

379

313

252

188

181

179

163

161

142

127

119

48

38

34

32

20

16

14

7

7

6

4

3

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Characteristics

Expected molecular weight of target: 136 kDa

Purification

Affinity purification

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Isotype

IgG

Top Product

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anti-Histone 3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivity: Human WB, IHC, ChIP, IP Host: Rabbit Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200, CHIP 1:20-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C.
Target

Histone 3 (H3)

Alternative Name

Histone H3 (H3 Products)

Synonyms

H-3 antibody, histocompatibility 3 antibody, H3 antibody

Molecular Weight

15 kDa

Gene ID

8290

UniProt

Q16695