TYSY (AA 265-294), (C-Term) antibody

Details for Product No. ABIN1881964, Supplier: Log in to see
Antigen
Epitope
C-Term, AA 265-294
5
3
Reactivity
Human
5
Host
Rabbit
5
Clonality (Clone)
Polyclonal ()
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
3
3
3
2
1
Options
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Immunogen This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.
Clone RB20041
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Background Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.

Synonyms: TYMS, TS, Thymidylate synthase
NCBI Accession NP_001062
UniProt P04818
Application Notes WB: 1:2000,IHC: 1:500,IF: 1:50,FACS: 1:50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
Western Blotting (WB) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Immunohistochemistry (IHC) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Immunohistochemistry (IHC) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Immunofluorescence (IF) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Immunofluorescence (IF) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Flow Cytometry (FACS) image for anti-TYSY (AA 265-294), (C-Term) antibody (ABIN1881964) TYSY Antibody (C-term) (ABIN1881964) western blot analysis in Hela,Jurkat and HL-60 c...
Product cited in: Ren, Kim, Koh, Chang, Eom, Yi, Seong, Kim, Bronner, Cho: "Comparative analysis of thymidylate synthase at the protein, mRNA, and DNA levels as prognostic markers in colorectal adenocarcinoma." in: Journal of surgical oncology, Vol. 100, Issue 7, pp. 546-52, 2009 (PubMed).

Schiffer, Clifton, Davisson, Santi, Stroud: "Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site." in: Biochemistry, Vol. 34, Issue 50, pp. 16279-87, 1996 (PubMed).

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