HRAS antibody (AA 111-189)

Catalog No. ABIN1903338

The Rabbit Polyclonal anti-HRAS antibody (ABIN1903338) specifically detects HRAS in WB, IHC, ICC and IHC (p). The antibody is reactive with Human and Rat samples.

Quick Overview for HRAS antibody (AA 111-189) (ABIN1903338)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HRAS Antibody

Binding Specificity: AA 111-189

Specificity: Human HRAS

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a region within amino acids 111 and 189 of H-Ras (SwissProt P01112).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IHC, IHC-P (7.5 μg/mL), WB (1:500 - 1:3000)

Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.0, 20 % glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS / H-Ras

Background: Name/Gene ID: HRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein

Gene ID: 3265

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling