PHGDH antibody (Internal Region)

Catalog No. ABIN1923534

Product Details anti-PHGDH Antibody

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PHGDH

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 209 and 437 of PHGDH (SwissProt O43175). Percent identity by BLAST analysis: Human (100%), Rat (97%), Mouse (96%), Porcine (90%), Bovine (83%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (7.5 μg/mL), WB (1:5000 - 1:20000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:5000-1:20000, IHC-Paraffin: 7.5 μg/mL [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH / PGDH (PHGDH Products)

Synonyms: fb38f06 antibody, zgc:65956 antibody, wu:fb38f06 antibody, embryo sac development arrest 9 antibody, F10M10.7 antibody, ECK2909 antibody, JW2880 antibody, 3-PGDH antibody, 3PGDH antibody, 4930479N23 antibody, A10 antibody, PGAD antibody, PGD antibody, PGDH antibody, SERA antibody, PDG antibody, phosphoglycerate dehydrogenase antibody, D-3-phosphoglycerate dehydrogenase antibody, 3-phosphoglycerate dehydrogenase antibody, phgdh antibody, EDA9 antibody, serA antibody, Sthe_2284 antibody, Dacet_1260 antibody, Mrub_0173 antibody, MMAH_RS07650 antibody, Arnit_0487 antibody, Ndas_0174 antibody, Mesil_2386 antibody, Slip_0010 antibody, Trad_0302 antibody, Acear_0027 antibody, Mahau_0665 antibody, MZHIL_RS05165 antibody, Mesop_1331 antibody, Thein_0855 antibody, Phgdh antibody, PHGDH antibody

Background: Name/Gene ID: PHGDH

Synonyms: PHGDH, 3-PGDH, 3PGDH, PGDH, Phosphoglycerate dehydrogenase, PDG, PGAD, PGDH3

Gene ID: 26227

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect