PPARG antibody (pSer273)

Catalog No. ABIN1925543

Product Details anti-PPARG Antibody

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Binding Specificity: pSer273

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPARG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: The antibody is directed against the phosphorylated form of human PPAR gamma at the S273 residue. A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100 % sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogen: Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000), WB (1 μg/mL)

Usage: Anti-PPAR antibody is useful for ELISA and Western Blot. Expect a band approximately ~57kD corresponding to the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute with 100 μL deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for PPARG

Target: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Alternative Name: PPARG / PPAR Gamma (PPARG Products)

Synonyms: PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody

Background: Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

Gene ID: 5468

Pathways: MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation