PTPN11 antibody (Tyr542)

Catalog No. ABIN197594

The Rabbit Polyclonal anti-PTPN11 antibody is suitable to detect PTPN11 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for PTPN11 antibody (Tyr542) (ABIN197594)

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTPN11 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PTPN11 Antibody

Binding Specificity: Tyr542

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human SHP-2 around the phosphorylation site of Tyrosine 542 (H-E-Yp-T-N).

Application Details

Application Notes: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PTPN11

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Alternative Name: PTPN11 / PTP2C

Background: SHP2 is a protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.Synonyms: PTP-1D, PTP-2C, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C, SH-PTP2, SH-PTP3, SHP-2, SHPTP2, Shp2, Tyrosine-protein phosphatase non-receptor type 11

Gene ID: 9606

UniProt: Q06124

Pathways: JAK-STAT Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effect