Chitotriosidase 1 antibody
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- Target See all Chitotriosidase 1 (CHIT1) Antibodies
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Chitotriosidase 1 antibody is un-conjugated
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Application
- ELISA, Immunoprecipitation (IP), Neutralization (Neut)
- Specificity
- Recognizes human CHIT1 in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhCHI-3L1. Species sequence homology: mouse: 79 % .
- Purification
- Protein G purified
- Immunogen
- NS0-derived rhCHIT-1.
- Isotype
- IgG1
- Top Product
- Discover our top product CHIT1 Primary Antibody
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- Application Notes
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Approved: ELISA, IP (25 μg/mL), Neut
Usage: Suitable for use in ELISA, Immunoprecipitation and Neutralization. ELISA (direct): 0.5-1.0 μg/mL. Detection limit for rhCHIT1 is approximately 2 ng/well. Immunoprecipitation: 25 μg/mL to immunoprecipitate rhCHIT1 from conditioned cell culture medium. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N, N', N''-triacetylchitotriose. 50 % of the enzymatic activity was inhibited by the antibody at approximately 56 ng/mL (IC 50) under conditions in which the enzyme was present at 20 ng/mL and the substrate concentration was 0.01 mM. IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
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- Target
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- Alternative Name
- CHIT1 / Chitotriosidase (CHIT1 Products)
- Synonyms
- CHI3 antibody, CHIT antibody, CHITD antibody, CHIT1 antibody, chio antibody, 2300002L19Rik antibody, chitinase 1 antibody, chitinase 1 (chitotriosidase) antibody, CHIT1 antibody, chit1 antibody, Chit1 antibody
- Background
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Name/Gene ID: CHIT1
Synonyms: CHIT1, CHITD, CHI3, Chitinase-1, Chitinase 1 (chitotriosidase), Chitotriosidase, CHIT, Chitotriosidase-1 - Gene ID
- 1118
- UniProt
- Q13231
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