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Vimentin antibody

VIM Reactivity: Human, Rat, Chicken WB, IHC, FACS, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal unconjugated
Catalog No. ABIN204914
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Reactivity
    • 333
    • 126
    • 125
    • 61
    • 58
    • 43
    • 42
    • 36
    • 29
    • 21
    • 20
    • 13
    • 13
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Chicken
    Host
    • 190
    • 129
    • 9
    • 6
    • 4
    • 4
    • 2
    Mouse
    Clonality
    • 198
    • 145
    Monoclonal
    Conjugate
    • 200
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 282
    • 132
    • 99
    • 98
    • 96
    • 65
    • 49
    • 40
    • 39
    • 28
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.
    Purification
    Protein G purified
    Immunogen
    Vimentin.

    Type of Immunogen: Purified protein
    Isotype
    IgG1
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes
    Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)

    Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer,  pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C,-20 °C
    Storage Comment
    4°C or -20°C, Avoid freeze-thaw cycles.
  • Target
    Vimentin (VIM)
    Alternative Name
    Vimentin (VIM Products)
    Synonyms
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    Background
    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin
    Gene ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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