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PRKAA1 antibody (AA 376-392)

PRKAA1 Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Monkey, Pig, Hamster WB, ELISA Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN210715

Datasheet as PDF

Target See all PRKAA1 Antibodies

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
Binding Specificity
29

17

16

15

8

8

7

6

6

5

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 376-392
Reactivity
147

126

82

24

9

7

7

7

6

6

5

5

4

3

2

2

1

1
Human, Mouse, Rat, Dog, Cow, Horse, Monkey, Pig, Hamster
Host
147

17

1
Rabbit
Compatible Secondaries
Clonality
137

28
Polyclonal
Conjugate
78

10

9

9

5

5

5

5

5

5

5

4

4

4

4

4

3

1
This PRKAA1 antibody is un-conjugated
Application
130

55

43

41

40

36

34

18

13

9

7

3

2

1

1
Western Blotting (WB), ELISA

Specificity

Recognizes human AMPK a1, Mr 63kD. Species cross-reactivity: rat and mouse.

Predicted Reactivity

Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus (94%) Xenopus, Zebrafish (88%) Pufferfish (82%).

Purification

Protein A purified

Immunogen

Synthetic peptide of human AMP-activated Protein Kinase a1 corresponding to aa 376-392 (C-ARHTLDELNPQKSKHQG-COOH) (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus (94%), Xenopus, Zebrafish (88%), Pufferfish (82%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype

IgG

Top Product

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anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 1-300) antibody
PRKAA1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 28-257) antibody
PRKAA1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 2B7 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pThr172), (pThr183) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat, Pig, Sheep WB, IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 351-450) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), FACS Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p), IF Host: Mouse Monoclonal 4D8 unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 325-543), (Catalytic Subunit alpha) antibody
PRKAA1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (pSer486) antibody
PRKAA1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-550) antibody
PRKAA1 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 4D9 unconjugated

Application Notes

Approved: ELISA, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name

PRKAA1 / AMPK Alpha 1 (PRKAA1 Products)

Synonyms

AMPK antibody, AMPKa1 antibody, cb116 antibody, im:7154392 antibody, sb:cb130 antibody, wu:fa94c10 antibody, AMPK1 antibody, AMPKalpha1 antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, C130083N04Rik antibody, ampk antibody, ampka1 antibody, PRKAA1 antibody, protein kinase AMP-activated catalytic subunit alpha 1 antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit antibody, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog antibody, PRKAA1 antibody, prkaa1 antibody, Prkaa1 antibody, prkaa1.L antibody

Background

Name/Gene ID: PRKAA1
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA1, ACACA kinase, AMPK alpha 1, AMPK alpha-1 chain, Acetyl-CoA carboxylase kinase, AMPKa1, AMPK subunit alpha-1, AMPK, AMPK1, Tau-protein kinase PRKAA1, HMGCR kinase

Gene ID

5562

UniProt

Q13131

Pathways

AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect