CTLA4 antibody

Catalog No. ABIN211607

Product Details anti-CTLA4 Antibody

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86. Is able to block ligand binding on the Raji B-cell line and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Further studies indicate that clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98,. The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2. CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface. CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases.

Purification: Affinity purified

Immunogen: Human CTLA-4/human IgG heavy chain fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:1 - 1:10), IHC, IHC-Fr (5 - 50 μg/mL)

Usage: Flow Cytometry: Membrane permeabilization is required for this application. Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4 / CD152 (CTLA4 Products)

Synonyms: CTLA-4 antibody, CTLA4 antibody, CD152 antibody, Cd152 antibody, Ctla-4 antibody, Ly-56 antibody, CD antibody, CELIAC3 antibody, GRD4 antibody, GSE antibody, IDDM12 antibody, sCTLA4 antibody, cytotoxic T-lymphocyte associated protein 4 antibody, cytotoxic T-lymphocyte-associated protein 4 antibody, CTLA4 antibody, Ctla4 antibody

Background: Name/Gene ID: CTLA4

Synonyms: CTLA4, CD152, CELIAC3, CD152 antigen, CTLA-4, Celiac disease 3, IDDM12, GRD4, GSE, CD152 isoform

Gene ID: 1493

Pathways: Cancer Immune Checkpoints