ERBB3 antibody (C-Term)
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- Target See all ERBB3 Antibodies
- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ERBB3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- Recognizes human HER3 at 180kD. Species cross-reactivity: mouse and rat. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum.
- Purification
- Protein A purified
- Immunogen
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A GST-HER3 fusion protein corresponding to aa1283 to 1323 at the carboxy-terminus of human HER3.
Type of Immunogen: Fusion protein - Isotype
- IgG
- Top Product
- Discover our top product ERBB3 Primary Antibody
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- Application Notes
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Approved: IP, WB
Usage: Suitable for use in Immunoprecipitation and Western Blot. Immunoprecipitation: 5 μL. Use 25 μL of Protein A-agarose beads and 1 mL of lysate (lysate contains approximately 1 mg of total protein). Rotate mixture overnight at 4?C and then wash 3x with lysis buffer. Dissolve the resulting bead complex in 20-30 μL of 3xSDS-PAGE sample buffer and load ~15 μL per lane on an 8 % polyacrylamide gel. Positive control: Human prostate cell lines (LNCap) or human breast cell line (MDA-453). - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
- Alternative Name
- HER3 / ERBB3 (ERBB3 Products)
- Synonyms
- ErbB-3 antibody, HER3 antibody, LCCS2 antibody, MDA-BF-1 antibody, c-erbB-3 antibody, c-erbB3 antibody, erbB3-S antibody, p180-ErbB3 antibody, p45-sErbB3 antibody, p85-sErbB3 antibody, ERBB3 antibody, C76256 antibody, Erbb-3 antibody, Erbb3r antibody, Her3 antibody, nuc-ErbB3 antibody, erb-b2 receptor tyrosine kinase 3 antibody, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody, ERBB3 antibody, Erbb3 antibody
- Background
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Name/Gene ID: ERBB3
Subfamily: EGF Receptor
Family: Protein Kinase
Synonyms: ERBB3, C-erbB-3, C-erbB3, ErbB-3, HER3, p85-sErbB3, p45-sErbB3, MDA-BF-1, ErbB3-S, LCCS2, p180-ErbB3 - Gene ID
- 2065
- UniProt
- P21860
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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