SPHK1 antibody (N-Term)

Catalog No. ABIN213425

Product Details anti-SPHK1 Antibody

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SPHK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (93%) Bat, Pig (87%) Mouse, Rat, Hamster (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from N-terminus of human SPHK1. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (93%), Bat, Pig (87%), Mouse, Rat, Hamster (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK / SPHK1 (SPHK1 Products)

Synonyms: CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody

Background: Name/Gene ID: SPHK1
Subfamily: Sphingosine
Family: Non-protein Kinase

Synonyms: SPHK1, SK1, Sphingosine kinase 1, SPHK, SK 1, SPK 1, Sphingosine kinase type 1, SPK

Gene ID: 8877

Pathways: VEGF Signaling