HIF1AN antibody (N-Term)
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- Target See all HIF1AN Antibodies
- HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))
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Binding Specificity
- N-Term
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Reactivity
- Human, Rabbit, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HIF1AN antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human HIF1AN
- Predicted Reactivity
- Percent identity by BLAST analysis: Human (92%) Rabbit, Horse (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human HIF1AN (Q9NWT6, NP_060372). Percent identity by BLAST analysis: Human (100%), Gorilla, Monkey (92%), Gibbon, Marmoset, Elephant, Rabbit, Horse (85%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product HIF1AN Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- HIF1AN (Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN))
- Alternative Name
- HIF1AN (HIF1AN Products)
- Synonyms
- FIH1 antibody, 2310046M24Rik antibody, A830014H24Rik antibody, cb728 antibody, id:ibd5139 antibody, hypoxia inducible factor 1 alpha subunit inhibitor antibody, hypoxia-inducible factor 1, alpha subunit inhibitor antibody, hypoxia inducible factor 1, alpha subunit inhibitor antibody, hypoxia-inducible factor 1, alpha subunit inhibitor S homeolog antibody, HIF1AN antibody, Hif1an antibody, hif1an antibody, hif1an.S antibody
- Background
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Name/Gene ID: HIF1AN
Synonyms: HIF1AN, Factor inhibiting HIF-1, FIH1, Factor inhibiting HIF1, FIH-1 - Gene ID
- 55662
- NCBI Accession
- NP_060372
- UniProt
- Q9NWT6
- Pathways
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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