POLR2B antibody (AA 805-854)
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- Target See all POLR2B Antibodies
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
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Binding Specificity
- AA 805-854
- Reactivity
- Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Pig, Xenopus laevis, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR2B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human POLR2B / RPB2
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product POLR2B Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- After adding water, will consist of PBS buffer with 2 % sucrose
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
- Alternative Name
- POLR2B / RPB2 (POLR2B Products)
- Synonyms
- Pol2rb antibody, Rpb140 antibody, Rpb2 antibody, POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, RNA polymerase II subunit B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, polymerase (RNA) II (DNA directed) polypeptide B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antibody, POLR2B antibody, polr2b antibody, LOC100640211 antibody, Polr2b antibody, polr2b.L antibody, rpb-2 antibody
- Background
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Name/Gene ID: POLR2B
Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140 - Gene ID
- 5431
- NCBI Accession
- NP_000929
- UniProt
- P30876
- Pathways
- Regulatory RNA Pathways, DNA Damage Repair
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