IL-6 antibody

Catalog No. ABIN2191748

Product Details anti-IL-6 Antibody

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), Immunoassay (IA), Detection (D)

Sterility: 0.2 μm filtered

Clone: MP5-32C11

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralisation of biological activity dilutions have to be made according to the amount of IL-6 to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Handling Advice: Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.

Storage: 4 °C

Storage Comment: Lyophilized product should be stored at 4 °C Store stock solution in aliquots at -20 °C Repeated freeze and thaw cycles will cause loss of activity. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-IL-6 antibody (ABIN2191748)

Hirano: "Interleukin 6 (IL-6) and its receptor: their role in plasma cell neoplasias." in: International journal of cell cloning, Vol. 9, Issue 3, pp. 166-84, (1991) (PubMed).

Hooghe-Peters, Velkeniers, Vanhaelst, Hooghe: "Interleukin-1, interleukin-6: messengers in the neuroendocrine immune system?" in: Pathology, research and practice, Vol. 187, Issue 5, pp. 622-5, (1991) (PubMed).

Takatsuki, Okano, Suzuki, Chieda, Takahara, Hirano, Kishimoto, Hamuro, Akiyama: "Human recombinant IL-6/B cell stimulatory factor 2 augments murine antigen-specific antibody responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 9, pp. 3072-7, (1988) (PubMed).

McKenzie: "Alloantigen presentation by B cells. Requirement for IL-1 and IL-6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 9, pp. 2907-11, (1988) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: Interleukin 6 (IL6 Products)

Synonyms: BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody

Background: The antibody neutralizes mouse interleukin 6 (IL-6). It does not cross-react with LPS (the component of the bacterial cell wall which is considered responsible for the toxicity of gram-negative bacteria) and TNF- alpha nor does it block human or rat IL-6 in proliferation assays using the KD83 cell line. MP5-32C11 inhibits mouse IL-6-induced proliferation of the NFS60 myelomonocytic cell line and KD83 plasmacytoma. IL-6 is a pluripotent 20-22 kDa cytokine which plays a role in the pathophysiology of severe infection and regulates the immune response, acute phase reaction and haematopoiesis. IL-6 plays a critical role in B- cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. Continuous IL-6 gene expression makes an essential contribution to a multistep oncogenesis of plasma cell neoplasia. IL-6 is a very useful culture supplement for the generation of a high number of antibody- producing hybridomas.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome