Tyrosine Hydroxylase antibody (C-Term)

Catalog No. ABIN6757539

Product Details anti-Tyrosine Hydroxylase Antibody

Target: Tyrosine Hydroxylase (TH)

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes an epitope within the catalytically-active C-terminal end-product. This C-terminal product is ~34kD. (This end product is the result of a partial digestion of the rat TH with chymotrypsin.) Species cross-reactivity: Human, primate, mouse.

Purification: Immunoaffinity purified

Immunogen: Rat tyrosine hydroxylase.

Isotype: IgM

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB (1:1000 - 1:2000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000-1:2000. Immunohistochemistry: 1:1000 -1:2000 in PBS/0.3 % Triton X-100 Cy3 Technique 1:4000-1:8000 in PBS/0.3 % Triton X-100 Bn-Av/HRP Technique. Control Tissue: Rat striatum and substantia nigra. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4. Post Fixation-1.5 hr. at 4? C in 4 % paraformaldehyde in 0.1 M Phosphate buffer,  pH 7.4. Sections: 10um cryostat frozen sections or 50um vibratome sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: TH / Tyrosine Hydroxylase (TH Products)

Synonyms: DYT14 antibody, DYT5b antibody, TYH antibody, The antibody, CG10118 antibody, DH65B antibody, DTH antibody, Dmel\\CG10118 antibody, Pale antibody, Ple antibody, TH antibody, Th antibody, VII antibody, dTH1 antibody, dTH65B antibody, pale/ple antibody, th antibody, dyt14 antibody, dyt5b antibody, tyh antibody, tyrosine hydroxylase antibody, pale antibody, tyrosine 3-monooxygenase antibody, tyrosine hydroxylase S homeolog antibody, TH antibody, Th antibody, ple antibody, th antibody, th.S antibody, LOC100008895 antibody

Background: Name/Gene ID: TH
Family: Monooxygenase

Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

Gene ID: 7054

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour