DBH antibody (AA 40-57)
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- Target See all DBH Antibodies
- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
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Binding Specificity
- AA 40-57
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DBH antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Recognizes Dopamine Beta Hydroxylase (DbH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of M. W. 70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species cross-reactivity: By immunoblot: human, monkey (slight), sheep, canine (slight), rat and bovine. Also shown to react with equine ganglia cells.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla (94%) Marmoset (89%) Mouse, Rat, Elephant, Porcine, Bovine (83%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide, C-SAPRESPLPYHIPLDPEG-amide, cloned from human (DbH) cDNA. Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla (94%), Marmoset (89%), Mouse, Rat, Elephant, Porcine, Bovine (83%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product DBH Primary Antibody
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- Application Notes
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Approved: IHC (1:250 - 1:1000), WB (1:250 - 1:1000)
Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:250-1:1000. Immunohistochemistry: 1:250-1:1000. Staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed weak staining on both human and monkey. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Dulbecco's PBS (divalent-deficient), 10 % glycerol, 10 mM HEPES, pH 7.2, 20 mM Tris-HCl, pH 7.6, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
- Alternative Name
- DBH/Dopamine Beta Hydroxylase (DBH Products)
- Synonyms
- CG1543 antibody, DBH antibody, Dbh antibody, Dmel\\CG1543 antibody, T beta h antibody, TBH antibody, TBetaH antibody, TBetah antibody, TBh antibody, TbetaH antibody, TbetaM antibody, Tbetah antibody, tbh antibody, DBM antibody, DOPBHY antibody, Tbh antibody, dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody, Tyramine beta hydroxylase antibody, MOXD1 homolog 2 antibody, dopamine beta hydroxylase antibody, dopamine beta-hydroxylase antibody, dbh antibody, Tbh antibody, LOC5574699 antibody, CpipJ_CPIJ004736 antibody, CpipJ_CPIJ004737 antibody, CpipJ_CPIJ014202 antibody, CpipJ_CPIJ016745 antibody, CpipJ_CPIJ019034 antibody, DBH antibody, Dbh antibody, LOC101110668 antibody
- Background
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Name/Gene ID: DBH
Family: Monooxygenase
Synonyms: DBH, Dopamine beta-monooxygenase, DBM, Dopamine beta-hydroxylase - Gene ID
- 1621
- UniProt
- P09172
- Pathways
- Carbohydrate Homeostasis
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