SMAD4 antibody

Catalog No. ABIN233840

Product Details anti-SMAD4 Antibody

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Mouse (Murine), Xenopus laevis

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human SMAD4 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 60 kDa in size corresponding to SMAD4 protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4 (SMAD4 Products)

Synonyms: smad4 antibody, DPC4 antibody, JIP antibody, MADH4 antibody, MYHRS antibody, AW743858 antibody, D18Wsu70e antibody, Madh4 antibody, XSmad4alpha antibody, Xsmad4 antibody, dpc4 antibody, jip antibody, madh4 antibody, xsmad4a antibody, SMAD family member 4 antibody, Smad4 protein antibody, SMAD family member 4, gene 1 L homeolog antibody, SMAD4 antibody, smad4 antibody, Smad4 antibody, smad4.1.L antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-b (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor.  SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription.  SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33.  In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus.   Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.
Synonyms: Deletion target in pancreatic carcinoma 4 antibody, DPC 4 antibody, MAD mothers against decapentaplegic homolog 4 antibody, MADH 4 antibody

Gene ID: 4089, 13603414

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy