The antibody Hs-14 reacts with a 220 kDa testis-specific human intra-acrosomal protein associated with the membranes of the acrosomal vesicle.
Characteristics
Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein
Immunogen
Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid,10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0. 2% AceticAcid and subsequently used for immunization.
ACRV1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Suitable for Flow Cytometry analysis of sperm cells using 20 μL reagent / 100 μL cellsuspension. The content of a vial (1 ml) is sufficient for 50 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2 % (w/v) high-grade BSA (protease free) as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody in the dark at 2-8 °C. DO NOT FREEZE! Avoid prolonged exposure to light. Shelf life: One year from despatch.
One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2