RFC4 antibody

Catalog No. ABIN2458986

Product Details anti-RFC4 Antibody

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC4.

Application Details

Application Notes: RFC4 antibody can be used for detection of RFC4 by ELISA at 1:62500. RFC4 antibody can be used for detection of RFC4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC4 antibody at -20 °C.

Target Details for RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Alternative Name: RFC4 (RFC4 Products)

Synonyms: 44.t00003 antibody, 21.m03053 antibody, rfc4 antibody, A1 antibody, AI894123 antibody, AU040575 antibody, RFC37 antibody, rfc2 antibody, replication factor C subunit 4 antibody, Replication factor C subunit 4 antibody, replication factor C (activator 1) 4 antibody, replication factor C subunit 4 L homeolog antibody, PFL2005w antibody, EHI_167150 antibody, TP03_0432 antibody, Tb11.01.1310 antibody, BBOV_IV002210 antibody, rfc4 antibody, rfc-4 antibody, Rfc4 antibody, RFC4 antibody, rfc4.L antibody

Background: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Molecular Weight: 40 kDa

Gene ID: 5984

NCBI Accession: NP_002907

UniProt: P35249

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA