Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

POLK antibody

POLK Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2459231
  • Target See all POLK Antibodies
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Reactivity
    • 55
    • 15
    • 13
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 47
    • 5
    • 3
    Rabbit
    Clonality
    • 52
    • 3
    Polyclonal
    Conjugate
    • 26
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLK antibody is un-conjugated
    Application
    • 29
    • 20
    • 14
    • 5
    • 4
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA
    Purification
    Antibody is purified by peptide affinity chromatography method.
    Immunogen
    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLK.
    Top Product
    Discover our top product POLK Primary Antibody
  • Application Notes
    POLK antibody can be used for detection of POLK by ELISA at 1:62500. POLK antibody can be used for detection of POLK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    Antibody is lyophilized in PBS buffer with 2 % sucrose.
    Handling Advice
    As with any antibody avoid repeat freeze-thaw cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    For short periods of storage (days) store at 4 °C. For longer periods of storage, store POLK antibody at -20 °C.
  • Target
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Alternative Name
    POLK (POLK Products)
    Synonyms
    DINB1 antibody, DINP antibody, POLQ antibody, Dinb1 antibody, POLK antibody, si:ch211-254o18.3 antibody, DNA polymerase kappa antibody, polymerase (DNA directed) kappa antibody, polymerase (DNA directed), kappa antibody, polymerase (DNA directed) kappa L homeolog antibody, POLK antibody, Polk antibody, polk antibody, Tb11.01.0080 antibody, Tb11.01.0010 antibody, Tb11.01.0020 antibody, Tb11.01.0030 antibody, Tb11.01.0040 antibody, Tb11.01.0050 antibody, Tb11.12.0001 antibody, Tb11.12.0002 antibody, Tb11.12.0003 antibody, polk.L antibody, polk-1 antibody
    Background
    POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
    Molecular Weight
    99 kDa
    Gene ID
    51426
    NCBI Accession
    NP_057302
    UniProt
    Q9UBT6
    Pathways
    DNA Damage Repair
You are here:
Support