Crossover junction endonuclease EME1 (EME1) antibody

Catalog No. ABIN2459612

Product Details

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EME1.

Application Details

Application Notes: EME1 antibody can be used for detection of EME1 by ELISA at 1:312500. EME1 antibody can be used for detection of EME1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EME1 antibody at -20 °C.

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1 (EME1 Products)

Synonyms: 6820428D13 antibody, essential meiotic structure-specific endonuclease 1 antibody, Eme1 antibody

Background: EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).

Molecular Weight: 63 kDa

Gene ID: 146956

NCBI Accession: NP_689676

UniProt: Q96AY2