LIG4 antibody

Catalog No. ABIN2460869

Product Details anti-LIG4 Antibody

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LIG4.

Application Details

Application Notes: LIG4 antibody can be used for detection of LIG4 by ELISA at 1:312500. LIG4 antibody can be used for detection of LIG4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store LIG4 antibody at -20 °C.

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4 (LIG4 Products)

Synonyms: zgc:165595 antibody, lig4 antibody, DNA LIGASE IV antibody, LIG4 antibody, lig4-A antibody, 5830471N16Rik antibody, tiny antibody, DNL4 antibody, ligase IV, DNA, ATP-dependent antibody, DNA ligase 4 antibody, ATP-dependent DNA ligase implicated in dsDNA break repair via nonhomologous end-joining antibody, DNA ligase IV antibody, ligase IV, DNA, ATP-dependent L homeolog antibody, lig4 antibody, LIG4 antibody, LOC100212302 antibody, CpipJ_CPIJ005161 antibody, PTRG_09982 antibody, MCYG_05151 antibody, LOC657210 antibody, ATLIG4 antibody, lig4.L antibody, Lig4 antibody

Background: LIG4 encodes a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V (D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.

Molecular Weight: 104 kDa

Gene ID: 3981

NCBI Accession: NP_002303

UniProt: Q8IY66

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response