MCM4 antibody
-
- Target See all MCM4 Antibodies
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
-
Reactivity
- Human, Mouse, Rat, Dog
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This MCM4 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.
- Top Product
- Discover our top product MCM4 Primary Antibody
-
-
- Application Notes
- MCM4 antibody can be used for detection of MCM4 by ELISA at 1:312500. MCM4 antibody can be used for detection of MCM4 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store MCM4 antibody at -20 °C.
-
- Target
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
- Alternative Name
- MCM4 (MCM4 Products)
- Synonyms
- cdc21 antibody, CDC21 antibody, CDC54 antibody, NKCD antibody, NKGCD antibody, P1-CDC21 antibody, hCdc21 antibody, 19G antibody, AI325074 antibody, AU045576 antibody, Cdc21 antibody, Mcmd4 antibody, mKIAA4003 antibody, mcdc21 antibody, cb1025 antibody, fc12c09 antibody, fj85g09 antibody, hm:zeh1616 antibody, wu:fc12c09 antibody, wu:fj85g09 antibody, zeh1616 antibody, minichromosome maintenance complex component 4 L homeolog antibody, minichromosome maintenance complex component 4 S homeolog antibody, minichromosome maintenance complex component 4 antibody, mcm4.L antibody, mcm4.S antibody, MCM4 antibody, mcm4 antibody, Mcm4 antibody
- Background
- The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
- Molecular Weight
- 95 kDa, 97 kDa
- Gene ID
- 4173
- NCBI Accession
- NP_877423
- UniProt
- P33991
- Pathways
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
-