eIF4EBP1 antibody (truncated)

Catalog No. ABIN2464007

The Mouse Monoclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Human. It has been validated for IHC.

Quick Overview for eIF4EBP1 antibody (truncated) (ABIN2464007)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 11G12C11

Product Details anti-eIF4EBP1 Antibody

Binding Specificity: truncated

Purification: Antibody is purified by protein A affinity chromatography.

Immunogen: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: IHC:1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: 4E-BP1 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4E-BP1

Background: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Gene ID: 1978

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling