CD14 antibody
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- Target See all CD14 Antibodies
- CD14
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD14 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA
- Purification
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Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml. - Immunogen
- Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
- Clone
- 5A3H5D5(FC)-5A3B11B5
- Isotype
- IgG1
- Top Product
- Discover our top product CD14 Primary Antibody
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- Application Notes
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IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified antibody in PBS containing 0.03 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Storage
- -20 °C
- Storage Comment
- CD14 monoclonal antibody can be stored at -20 °C, stable for one year.
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Synonyms
- CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
- Background
- CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
- Gene ID
- 929
- UniProt
- P08571
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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