IQGAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
IQGAP1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of IQGAP1.
IQGAP1
Reactivity: Human, Mouse, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 200 kDa band observed in human kidney and mouse kidney lysates (calculated MW of 189 kDa according to NP_003861.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
IQGAP1
(IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))