Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognise all reported Human isoforms according to NP_037390.2 and NP_660183.1.
Purification
PYCARD antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PYCARD antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of PYCARD.
PYCARD
Reactivity: Human, Mouse, Rat
IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 22 kDa band observed in Hela and human kidney lysates (predicted MW of 22 kDa according to NP_037390.2). Recommended for use at 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows staining of sinusoidal lining cells. Recommended concentration, 2-4μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.