Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
Both variants (NP_006057.1and NP_697021.1) represent identical protein
Purification
Aldehyde Reductase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Aldehyde Reductase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Aldehyde Reductase.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 38 kDa band observed in human placenta and liver lysates (calculated MW of 36.6 kDa according to NP_006057.1). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows Brush Border staining in PCT. Recommended concentration, 2-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
AKR1B1
(Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))