ENPEP antibody (Internal Region)

Catalog No. ABIN2465399

Product Details anti-ENPEP Antibody

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPEP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Aminopeptidase A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Aminopeptidase A antibody was raised against a 14 amino acid synthetic peptide near the internal region of Aminopeptidase A.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 170 kDa band observed in human brain and testis lysates (calculated MW of 109 kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May,87 (5) :2303-9, PMID: 11994381). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration, 3-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: Aminopeptidase A (ENPEP Products)

Synonyms: im:7152184 antibody, si:ch211-146m5.2 antibody, 6030431M22Rik antibody, APA antibody, Bp-1/6C3 antibody, Ly-51 antibody, Ly51 antibody, CD249 antibody, gp160 antibody, glutamyl aminopeptidase antibody, ENPEP antibody, enpep antibody, ECU01_1470 antibody, pepA antibody, LOC5568916 antibody, CpipJ_CPIJ011103 antibody, Eint_011390 antibody, Halhy_0222 antibody, Enpep antibody

Gene ID: 2028

NCBI Accession: NP_001968

UniProt: Q07075

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones