Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
Purification
DDX5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DDX5 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of DDX5.
DDX5
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 70 kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1 kDa according to NP_004387). Recommended concentration: 0.11-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.