Fibulin 5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Fibulin 5 antibody was raised against a 14 amino acid synthetic peptide near the internal region (near the C-Terminus) of Fibulin 5.
FBLN5
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 50 kDa band observed in human colon, heart and ovary lysates (calculated MW of 50.2 kDa according to NP_006320.2). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 23 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.