GDF15 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GDF15 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of GDF15.
GDF15
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 35 kDa band observed in human prostate lysates (calculated MW of 34.1 kDa according to NP_004855.1). In transfected HEK293 transiently expressing GDF15 a band of approximately 35 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.